Molecular characterization, tissue expression, and antiviral activities of Bama minipig interferon-α subtypes
Aziz Ullah Noor,
Lu Huipeng,
Zhanyu Du,
Song Chengyi,
Zhou Xiaohui,
Liu Xiaoming,
Suliman khan,
Huaichang Sun,
Abdelouahab Bellou
Affiliations
Aziz Ullah Noor
Institute of Sciences in Emergency Medicine Department of Emergency Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, Guangzhou, 510080, Guangdong Province, China; The College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China; Corresponding author. Institute of Sciences in Emergency Medicine Department of Emergency Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, Guangzhou, 510080, Guangdong Province, China.
Lu Huipeng
The College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China
Zhanyu Du
The College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China
Song Chengyi
The College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China
Zhou Xiaohui
The College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China
Liu Xiaoming
The College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China
Suliman khan
Department of Vet. Physiology and Biochemistry, FVAS, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, Balochistan, Pakistan
Huaichang Sun
The College of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China
Abdelouahab Bellou
Institute of Sciences in Emergency Medicine Department of Emergency Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, Guangzhou, 510080, Guangdong Province, China; Southern Medical University, Guangzhou, Guangdong, 510080, China; Department of Pediatrics, College of Medicine and Health Sciences, UAE University, Al Ain, P.O. BOX 15551, United Arab Emirates; Global Network on Emergency Medicine, Brookline, MA, 02446, USA; Corresponding author. Institute of Sciences in Emergency Medicine Department of Emergency Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, Guangzhou, 510080, Guangdong Province, China.
Interferons play a major role in innate immunity and disease resistance. Porcine interferon alpha has 17 subtypes, and their gene sequences, tissue expression profiles, and antiviral activities have been primarily studied in domestic pigs but not in minipigs. Bama minipigs are genetically stable disease-resistant and making them as laboratory animal models for bioscience studies. To define the potential mechanism for disease resistance, in this study, we cloned 17 subtypes of Porcine interferon alpha genes in Bama minipigs using high fidelity polymerase chain reaction and subsequent sequencing. Sequence alignment showed that the 17 porcine interferon alpha subtypes were 98%–100 % homologous in those of domestic pigs. However, significantly different tissue expression profiles of PoIFN-α subtypes were found in the two pig species using real-time quantitative RT-PCR. Among the 10 different Bama minipig tissues tested, significant expression of multi-subtype porcine interferon alpha was detected in the lymph nodes and spleen, whereas no or low expression of fewer subtypes was detected in the heart, lung, brain, and small intestine. Sequence analysis revealed that the porcine interferon alpha promoters were almost similar between the two pig species. A cytopathic effect inhibition assay showed that the recombinant 17 porcine interferon alpha subtypes purified from mammalian cells had significantly different antiviral profile against vesicular stomatitis virus, porcine pseudorabies virus and porcine reproductive and respiratory syndrome virus compared with those in domestic pigs. Our findings provide evidence that porcine interferon alpha subtypes are highly conserved between Bama minipigs and domestic pigs but show varied tissue expression pattern and antiviral capabilities, which may contribute to their differences in disease resistance.
