New Gene Markers Involved in Molecular Processes of Tissue Repair, Response to Wounding and Regeneration Are Differently Expressed in Fibroblasts from Porcine Oral Mucosa during Long-Term Primary Culture
Artur Bryja,
Patrycja Sujka-Kordowska,
Aneta Konwerska,
Sylwia Ciesiółka,
Maria Wieczorkiewicz,
Dorota Bukowska,
Paweł Antosik,
Rut Bryl,
Mariusz T. Skowroński,
Jędrzej M. Jaśkowski,
Paul Mozdziak,
Ana Angelova Volponi,
Jamil A. Shibli,
Bartosz Kempisty,
Marta Dyszkiewicz-Konwińska
Affiliations
Artur Bryja
Department of Anatomy, Poznan University of Medical Science, 60-781 Poznań, Poland
Patrycja Sujka-Kordowska
Department of Histology and Embryology, Poznan University of Medical Science, 60-781 Poznań, Poland
Aneta Konwerska
Department of Histology and Embryology, Poznan University of Medical Science, 60-781 Poznań, Poland
Sylwia Ciesiółka
Department of Histology and Embryology, Poznan University of Medical Science, 60-781 Poznań, Poland
Maria Wieczorkiewicz
Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland
Dorota Bukowska
Department of Diagnostics and Clinical Sciences, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland
Paweł Antosik
Department of Veterinary Surgery, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland
Rut Bryl
Department of Anatomy, Poznan University of Medical Science, 60-781 Poznań, Poland
Mariusz T. Skowroński
Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland
Jędrzej M. Jaśkowski
Department of Diagnostics and Clinical Sciences, Nicolaus Copernicus University in Torun, 87-100 Toruń, Poland
Paul Mozdziak
Physiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA
Ana Angelova Volponi
Department of Craniofacial Development and Stem Cell Biology, King’s College University of London, London WC2R 2LS, UK
Jamil A. Shibli
Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos, Guarulhos SP 07030-010, Brazil
Bartosz Kempisty
Department of Anatomy, Poznan University of Medical Science, 60-781 Poznań, Poland
Marta Dyszkiewicz-Konwińska
Department of Anatomy, Poznan University of Medical Science, 60-781 Poznań, Poland
The mechanisms of wound healing and vascularization are crucial steps of the complex morphological process of tissue reconstruction. In addition to epithelial cells, fibroblasts play an important role in this process. They are characterized by dynamic proliferation and they form the stroma for epithelial cells. In this study, we have used primary cultures of oral fibroblasts, obtained from porcine buccal mucosa. Cells were maintained long-term in in vitro conditions, in order to investigate the expression profile of the molecular markers involved in wound healing and vascularization. Based on the Affymetrix assays, we have observed three ontological groups of markers as wound healing group, response to wounding group and vascularization group, represented by different genes characterized by their expression profile during long-term primary in vitro culture (IVC) of porcine oral fibroblasts. Following the analysis of gene expression in three previously identified groups of genes, we have identified that transforming growth factor beta 1 (TGFB1), ITGB3, PDPN, and ETS1 are involved in all three processes, suggesting that these genes could be recognized as markers of repair specific for oral fibroblasts within the porcine mucosal tissue.
