Transcriptome and Metabolome Integrated Analysis of Two Ecotypes of <i>Tetrastigma hemsleyanum</i> Reveals Candidate Genes Involved in Chlorogenic Acid Accumulation
Shuya Yin,
Hairui Cui,
Le Zhang,
Jianli Yan,
Lihua Qian,
Songlin Ruan
Affiliations
Shuya Yin
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Hairui Cui
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Le Zhang
Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310058, China
Jianli Yan
Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310058, China
Lihua Qian
Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310058, China
Songlin Ruan
Institute of Crops, Hangzhou Academy of Agricultural Sciences, Hangzhou 310058, China
T. hemsleyanum plants with different geographical origins contain enormous genetic variability, which causes different composition and content of flavonoids. In this research, integrated analysis of transcriptome and metabolome were performed in two ecotypes of T. hemsleyanum. There were 5428 different expressed transcripts and 236 differentially accumulated metabolites, phenylpropane and flavonoid biosynthesis were most predominantly enriched. A regulatory network of 9 transcripts and 11 compounds up-regulated in RG was formed, and chlorogenic acid was a core component.
