Carbon source and gelling agent influences on Indica rice regeneration (cv IACuba-28) from callus

Abstract

Several variations on plant regeneration medium from callus in IACuba-28 rice cultivar were evaluated using the basal MS medium with the same grouth regulator concentrations. As carbon source 30g.l-1 of maltose or sucrose was used, in combination with 8g.l-1 of agarose or 4.3g.l-1 of phytagel as gelling agent. In all cases green shoots in callus were observed, but the best results were obtained using the combination maltose-phytagel, where the first green shoots were detected and the higher plant number was obtained five days after callus incubation in a shorter time. Key words: agarose, maltose, shoots