Influence of growth regulators on plant regeneration in tomato

Abstract

We studied the effect of different plant growth regulators on in vitro regeneration and plant growth of three cultivars of tomato (Lycopersicon esculentum Mill.) from explants derived from hypocotyls and cotyledons of aseptically grown seedlings. The regeneration capacity was significantly influenced by cultivar and explant type. The highest number of shoots regenerated in both types of explants was recorded on MS medium supplemented with 1.0 mg/dm3 zeatin and 0.1 mg/dm3 IAA. The cultivar UC 82 showed the best regeneration capacity on all types of used media. The most responsive explants were hypocotyls with 90-92% regeneration in dependence on the used cultivars and mean production from 0.18 to 0.38 shoots per explant.

Keywords

• culture in vitro
• organogenesis
• bap
• iaa
• tdz
• zea
• lycopersicon esculentum