Frontiers in Microbiology (Feb 2024)
Genomic and ecological approaches to identify the Bifidobacterium breve prototype of the healthy human gut microbiota
- Chiara Argentini,
- Gabriele Andrea Lugli,
- Gabriele Andrea Lugli,
- Chiara Tarracchini,
- Federico Fontana,
- Federico Fontana,
- Leonardo Mancabelli,
- Leonardo Mancabelli,
- Alice Viappiani,
- Rosaria Anzalone,
- Leonora Angelini,
- Giulia Alessandri,
- Giulia Longhi,
- Massimiliano G. Bianchi,
- Massimiliano G. Bianchi,
- Giuseppe Taurino,
- Giuseppe Taurino,
- Ovidio Bussolati,
- Ovidio Bussolati,
- Christian Milani,
- Christian Milani,
- Douwe van Sinderen,
- Francesca Turroni,
- Francesca Turroni,
- Marco Ventura,
- Marco Ventura
Affiliations
- Chiara Argentini
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Gabriele Andrea Lugli
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Gabriele Andrea Lugli
- Microbiome Research Hub, University of Parma, Parma, Italy
- Chiara Tarracchini
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Federico Fontana
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Federico Fontana
- GenProbio srl, Parma, Italy
- Leonardo Mancabelli
- Microbiome Research Hub, University of Parma, Parma, Italy
- Leonardo Mancabelli
- Department of Medicine and Surgery, University of Parma, Parma, Italy
- Alice Viappiani
- GenProbio srl, Parma, Italy
- Rosaria Anzalone
- GenProbio srl, Parma, Italy
- Leonora Angelini
- GenProbio srl, Parma, Italy
- Giulia Alessandri
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Giulia Longhi
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Massimiliano G. Bianchi
- Microbiome Research Hub, University of Parma, Parma, Italy
- Massimiliano G. Bianchi
- Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
- Giuseppe Taurino
- Microbiome Research Hub, University of Parma, Parma, Italy
- Giuseppe Taurino
- Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
- Ovidio Bussolati
- Microbiome Research Hub, University of Parma, Parma, Italy
- Ovidio Bussolati
- Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
- Christian Milani
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Christian Milani
- Microbiome Research Hub, University of Parma, Parma, Italy
- Douwe van Sinderen
- APC Microbiome Institute and School of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland
- Francesca Turroni
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Francesca Turroni
- Microbiome Research Hub, University of Parma, Parma, Italy
- Marco Ventura
- Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy
- Marco Ventura
- Microbiome Research Hub, University of Parma, Parma, Italy
- DOI
- https://doi.org/10.3389/fmicb.2024.1349391
- Journal volume & issue
-
Vol. 15
Abstract
Members of the genus Bifidobacterium are among the first microorganisms colonizing the human gut. Among these species, strains of Bifidobacterium breve are known to be commonly transmitted from mother to her newborn, while this species has also been linked with activities supporting human wellbeing. In the current study, an in silico approach, guided by ecology- and phylogenome-based analyses, was employed to identify a representative strain of B. breve to be exploited as a novel health-promoting candidate. The selected strain, i.e., B. breve PRL2012, was found to well represent the genetic content and functional genomic features of the B. breve taxon. We evaluated the ability of PRL2012 to survive in the gastrointestinal tract and to interact with other human gut commensal microbes. When co-cultivated with various human gut commensals, B. breve PRL2012 revealed an enhancement of its metabolic activity coupled with the activation of cellular defense mechanisms to apparently improve its survivability in a simulated ecosystem resembling the human microbiome.
Keywords
