Brazilian Journal of Veterinary Medicine (Jan 2020)
PARÂMETROS HISTOLÓGICOS INTESTINAIS E IMUNOLÓGICOS DE FRANGOS DE CORTE SUPLEMENTADOS COM PROBIÓTICO COMERCIAL OU FUNGOS DA MICROBIOTA AUTÓCTONE
Abstract
The objective of this study was to evaluate the histomorphometry of intestinal portions of broilers supplemented with probiotic, as well as histological parameters of organs linked to the immune system. The evaluated fungus Rhizomucor spp. is from samples of the intestinal contents of chickens Cobb 500. This was selected in vitro as probiotic characteristics and routed to performance assay. A completely randomized design with 3 treatments and 7 replicates and 18 birds per unit was used. One bird per experimental unit was sacrificed and the organs were sampled, after fixation were submitted to dehydration and diaphanization and then analyzed by light microscopy and submitted to histomorphometry and searched for spots. It was observed at 21 days that the birds belonging to the probiotic group formulated with Rhizomucor spp. presented higher villus height in the duodenum. There was also a significant increase in jejunum villus height for the control and probiotic group formulated with Rhizomucor spp. At 42 days it was observed only difference to depth of crypt with commercial probiotic use for duodenum. Regarding the analyzes of the tissue changes of the intestines, spleen and bursa at 21 days, one hundred percent of the birds receiving the fungus Rhizomucor spp. did not present histological alterations and at 42 days bursa alterations were observed in two animals. The inclusion of probiotic probiotic potential Rhizomucor spp. in the diet of broilers at 21 days of age promotes higher height of villi and at 42 days of age there was no influence.
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