Macrophages-derived high-mobility group box-1 protein induces endothelial progenitor cells pyroptosis
Menghao Zeng,
Guibin Liang,
Fangfang Yuan,
Shanshan Yan,
Jie Liu,
Zhihui He
Affiliations
Menghao Zeng
Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China; Sepsis Translational Medicine Key Laboratory of Hunan Province, Changsha, Hunan, China
Guibin Liang
Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China; Sepsis Translational Medicine Key Laboratory of Hunan Province, Changsha, Hunan, China
Fangfang Yuan
Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China
Shanshan Yan
Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China
Jie Liu
Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China
Zhihui He
Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China; Sepsis Translational Medicine Key Laboratory of Hunan Province, Changsha, Hunan, China; National Engineering Research Center for Human Stem Cells, Changsha, Hunan, China; Corresponding author
Summary: Endothelial dysfunction is an important factor in the progress of sepsis. Endothelial progenitor cells (EPCs) are the precursor cells of endothelial cells and play a crucial role in the prognosis and treatment of sepsis. EPCs in the peripheral blood of patients with sepsis undergo pyroptosis, but the mechanism remains much of unknown. Serum high-mobility group box-1 (HMGB1) is significantly elevated in patients with sepsis, but whether it is related to EPCs pyroptosis is unknown. We used a cell model of sepsis in vitro to isolate EPCs for better observation. By detecting the pyroptosis-related indicators of EPCs and the level of release and acetylation of HMGB1 in inflammatory macrophages, it was found that HMGB1 released by inflammatory macrophages combined with receptor for advanced glycation end products (RAGE) is a key pathway to induce pyroptosis of EPCs.
