Frontiers in Medicine (Mar 2022)
From Macroscopy to Ultrastructure: An Integrative Approach to Pulmonary Pathology
- Stijn E. Verleden,
- Stijn E. Verleden,
- Stijn E. Verleden,
- Peter Braubach,
- Peter Braubach,
- Christopher Werlein,
- Edith Plucinski,
- Edith Plucinski,
- Mark P. Kuhnel,
- Mark P. Kuhnel,
- Annemiek Snoeckx,
- Haroun El Addouli,
- Tobias Welte,
- Tobias Welte,
- Axel Haverich,
- Axel Haverich,
- Florian P. Laenger,
- Florian P. Laenger,
- Sabine Dettmer,
- Sabine Dettmer,
- Patrick Pauwels,
- Veronique Verplancke,
- Paul E. Van Schil,
- Paul E. Van Schil,
- Therese Lapperre,
- Therese Lapperre,
- Johanna M. Kwakkel-Van-Erp,
- Johanna M. Kwakkel-Van-Erp,
- Maximilian Ackermann,
- Maximilian Ackermann,
- Jeroen M. H. Hendriks,
- Jeroen M. H. Hendriks,
- Danny Jonigk,
- Danny Jonigk
Affiliations
- Stijn E. Verleden
- Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), Antwerp University, Antwerp, Belgium
- Stijn E. Verleden
- Division of Pneumology, University Hospital Antwerp, Edegem, Belgium
- Stijn E. Verleden
- Department of Thoracic and Vascular Surgery, University Hospital Antwerp, Edegem, Belgium
- Peter Braubach
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Peter Braubach
- Institute for Pathology, Hannover Medical School, Hannover, Germany
- Christopher Werlein
- Institute for Pathology, Hannover Medical School, Hannover, Germany
- Edith Plucinski
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Edith Plucinski
- Institute for Pathology, Hannover Medical School, Hannover, Germany
- Mark P. Kuhnel
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Mark P. Kuhnel
- Institute for Pathology, Hannover Medical School, Hannover, Germany
- Annemiek Snoeckx
- Division of Radiology, University Hospital Antwerp and University of Antwerp, Edegem, Belgium
- Haroun El Addouli
- Division of Radiology, University Hospital Antwerp and University of Antwerp, Edegem, Belgium
- Tobias Welte
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Tobias Welte
- Division of Pneumology, Hannover Medical School, Hannover, Germany
- Axel Haverich
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Axel Haverich
- Division of Thoracic Surgery, Hannover Medical School, Hannover, Germany
- Florian P. Laenger
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Florian P. Laenger
- Institute for Pathology, Hannover Medical School, Hannover, Germany
- Sabine Dettmer
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Sabine Dettmer
- Department of Radiology, Hannover Medical School, Hannover, Germany
- Patrick Pauwels
- 0Division of Pathology, University Hospital Antwerp, Edegem, Belgium
- Veronique Verplancke
- Division of Pneumology, University Hospital Antwerp, Edegem, Belgium
- Paul E. Van Schil
- Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), Antwerp University, Antwerp, Belgium
- Paul E. Van Schil
- Department of Thoracic and Vascular Surgery, University Hospital Antwerp, Edegem, Belgium
- Therese Lapperre
- Division of Pneumology, University Hospital Antwerp, Edegem, Belgium
- Therese Lapperre
- 1Laboratory of Experimental Medicine and Pediatrics (LEMP), Antwerp University, Antwerp, Belgium
- Johanna M. Kwakkel-Van-Erp
- Division of Pneumology, University Hospital Antwerp, Edegem, Belgium
- Johanna M. Kwakkel-Van-Erp
- 1Laboratory of Experimental Medicine and Pediatrics (LEMP), Antwerp University, Antwerp, Belgium
- Maximilian Ackermann
- 2Institute of Pathology and Department of Molecular Pathology, Helios University Clinic Wuppertal, University of Witten-Herdecke, Witten, Germany
- Maximilian Ackermann
- 3Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
- Jeroen M. H. Hendriks
- Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), Antwerp University, Antwerp, Belgium
- Jeroen M. H. Hendriks
- Department of Thoracic and Vascular Surgery, University Hospital Antwerp, Edegem, Belgium
- Danny Jonigk
- Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany
- Danny Jonigk
- Institute for Pathology, Hannover Medical School, Hannover, Germany
- DOI
- https://doi.org/10.3389/fmed.2022.859337
- Journal volume & issue
-
Vol. 9
Abstract
Pathology and radiology are complimentary tools, and their joint application is often crucial in obtaining an accurate diagnosis in non-neoplastic pulmonary diseases. However, both come with significant limitations of their own: Computed Tomography (CT) can only visualize larger structures due to its inherent–relatively–poor resolution, while (histo) pathology is often limited due to small sample size and sampling error and only allows for a 2D investigation. An innovative approach of inflating whole lung specimens and subjecting these subsequently to CT and whole lung microCT allows for an accurate matching of CT-imaging and histopathology data of exactly the same areas. Systematic application of this approach allows for a more targeted assessment of localized disease extent and more specifically can be used to investigate early mechanisms of lung diseases on a morphological and molecular level. Therefore, this technique is suitable to selectively investigate changes in the large and small airways, as well as the pulmonary arteries, veins and capillaries in relation to the disease extent in the same lung specimen. In this perspective we provide an overview of the different strategies that are currently being used, as well as how this growing field could further evolve.
Keywords
