Single-Atom Ce-N4-C-(OH)2 Nanozyme-Catalyzed Cascade Reaction to Alleviate Hyperglycemia
Guangchun Song,
Jia Xu,
Hong Zhong,
Qi Zhang,
Xin Wang,
Yitong Lin,
Scott P. Beckman,
Yunbo Luo,
Xiaoyun He,
Jin-Cheng Li,
Kunlun Huang,
Nan Cheng
Affiliations
Guangchun Song
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Jia Xu
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Hong Zhong
School of Mechanical and Materials Engineering,
Washington State University, Pullman, WA 99164, USA.
Qi Zhang
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Xin Wang
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Yitong Lin
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Scott P. Beckman
School of Mechanical and Materials Engineering,
Washington State University, Pullman, WA 99164, USA.
Yunbo Luo
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Xiaoyun He
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Jin-Cheng Li
Faculty of Chemical Engineering, Yunnan Provincial Key Laboratory of Energy Saving in Phosphorus, Chemical Engineering and New Phosphorus Materials,
Kunming University of Science and Technology, Kunming 650000, China.
Kunlun Huang
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
Nan Cheng
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering,
China Agricultural University, Beijing 100083, China.
The enzyme-mimicking catalytic activity of single-atom nanozymes has been widely used in tumor treatment. However, research on alleviating metabolic diseases, such as hyperglycemia, has not been reported. Herein, we found that the single-atom Ce-N4-C-(OH)2 (SACe-N4-C-(OH)2) nanozyme promoted glucose absorption in lysosomes, resulting in increased reactive oxygen species production in HepG2 cells. Furthermore, the SACe-N4-C-(OH)2 nanozyme initiated a cascade reaction involving superoxide dismutase-, oxidase-, catalase-, and peroxidase-like activity to overcome the limitations associated with the substrate and produce •OH, thus improving glucose intolerance and insulin resistance by increasing the phosphorylation of protein kinase B and glycogen synthase kinase 3β, and the expression of glycogen synthase, promoting glycogen synthesis to improve glucose intolerance and insulin resistance in high-fat diet-induced hyperglycemic mice. Altogether, these results demonstrated that the novel nanozyme SACe-N4-C-(OH)2 alleviated the effects of hyperglycemia without evident toxicity, demonstrating its excellent clinical application potential.
