Whole genome sequence of Salmonella Rissen SCSW714, a porcine strain harbouring a novel multidrug-resistant Tn7-like pco- and sil-containing transposon

Abstract

ABSTRACT: Objectives: The aim of this study was to characterise the whole genome sequence of multidrug resistance (MDR) Salmonella Rissen strain SCSW714 of swine origin. Methods: The whole genome of SCSW714 was sequenced using the Illumina Hiseq platform combined with the Nanopore PromethION platform and assembled by software Unicycler. NCBI Prokaryotic Genome Annotation Pipeline was used to annotate the genome of SCSW714. The sequence type (ST) as well as antimicrobial resistance genes were determined by MLST 2.0 and ResFinder 4.1, respectively. Results: The chromosome of SCSW714 was 4 928 262 bp in size with a GC content of 52.1%. Strain SCSW714 contained a total of 4759 genes, including 4531 protein-coding sequences, 108 pseudogenes and 120 RNAs. It belonged to ST469 and carried six resistance genes including tet(A), dfrA12, sul3, aadA2, aadA1 and blaTEM-1b. All of the six resistance genes were carried by a novel MDR Tn7-pco-sil transposon designated as Tn6777. Tn6777 was stable in S. Rissen and could be excised from S. Rissen chromosome. Conclusion: We report a complete genome sequence of S. Rissen and characterised a novel MDR Tn7-like pco- and sil-containing transposon for the first time. The excision of Tn6777 suggests that Tn6777 has functional activity and may promote the co-spreading of metal and antimicrobial resistance genes. The complete genome sequence of S. Rissen strain SCSW714 provides valuable information for tracing the potential spread from swine to humans.

Keywords

• Salmonella Rissen
• Multidrug resistance
• Metal resistance
• Tn7