مجله علوم پزشکی فیض (پیوسته) (Oct 2024)
Effect of 8-week resistance training combined with Tribulus terrestris extract on MDA, AOPP, and IL-10 levels in the heart tissue of male Wistar rats exposed to stanozolol
Abstract
Background and Aim: The aim of this study was to determine the effect of 8 weeks of resistance training combined with Tribulus terrestris extract on oxidative stress and pro-inflammatory cytokine markers in the heart tissue of male Wistar rats exposed to stanozolol. Methods: In this experimental study, 48 male Sprague-Dawley rats, aged 8 weeks, were randomly divided into 8 groups of 6 (1. Control, 2. Sham, 3. Stanozolol, 4. Stanozolol + Tribulus 50 mg/kg, 5. Stanozolol + Tribulus 100 mg/kg, 6. Resistance training, 7. Stanozolol + Tribulus 100 mg/kg + Resistance training, 8. Stanozolol + Tribulus 50 mg/kg + Resistance training). For 8 weeks, groups 3 to 8 received 5 mg/kg stanozolol daily via intraperitoneal injection. Groups 6 to 8 performed resistance training three times a week, and groups 4, 5, 7, and 8 received the specified doses of Tribulus terrestris daily via intraperitoneal injection. The expression levels of genes of Interleukin-10 (IL-10), Malondialdehyde (MDA), and Advanced Oxidation Protein Products (AOPP) were measured using Real-Time PCR. Results: Stanozolol significantly increased AOPP and MDA expression and decreased IL-10 expression in the heart tissue of rats (P<0.05). However, stanozolol combined with training significantly increased IL-10 and decreased AOPP and MDA (P<0.05). Additionally, stanozolol combined with training and Tribulus 50 and 100 mg/kg significantly increased IL-10 and decreased MDA and AOPP (P<0.05). In the group of stanozolol combined with training and Tribulus 100 mg/kg, IL-10 levels were higher and MDA levels were lower compared to the groups of stanozolol combined with training and stanozolol combined with training and Tribulus 50 mg/kg, but there was no significant difference in AOPP. Conclusion: Resistance training and Tribulus terrestris extract appear to be mitigating factors for oxidative stress and inflammation in the heart tissue of rats, and the dose of 100 mg/kg had a greater effect on improving oxidative stress and pro-inflammatory markers in the heart tissue.
