Emerging Infectious Diseases (Feb 2006)

Sequencing and Staphylococci Identification

  • Alexander Mellmann,
  • Karsten Becker,
  • Christof von Eiff,
  • Ursula Keckevoet,
  • Peter Schumann,
  • Dag Harmsen

DOI
https://doi.org/10.3201/eid1202.050962
Journal volume & issue
Vol. 12, no. 2
pp. 333 – 336

Abstract

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The emerging clinical importance of staphylococcal infections prompted us to establish a reference database for partial RNA polymerase B (rpoB; nucleotides 1444–1928) gene sequences from type strains of all staphylococcal species and subspecies. This database correctly identified 55 clinical staphylococcal isolates; all were correctly identified at the species level. At the subspecies level, rpoB misidentified only 2 isolates.

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