Frontiers in Veterinary Science (Apr 2022)
Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes
- Liyi Chen,
- Liyi Chen,
- Xinheng Zhang,
- Xinheng Zhang,
- Xinheng Zhang,
- Guanming Shao,
- Guanming Shao,
- Yangyang Shao,
- Yangyang Shao,
- Zezhong Hu,
- Zezhong Hu,
- Keyu Feng,
- Keyu Feng,
- Keyu Feng,
- Zi Xie,
- Zi Xie,
- Hongxin Li,
- Hongxin Li,
- Hongxin Li,
- Weiguo Chen,
- Weiguo Chen,
- Weiguo Chen,
- Wencheng Lin,
- Wencheng Lin,
- Wencheng Lin,
- Hengxing Yuan,
- Hailong Wang,
- Jun Fu,
- Qingmei Xie,
- Qingmei Xie,
- Qingmei Xie
Affiliations
- Liyi Chen
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Liyi Chen
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Xinheng Zhang
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xinheng Zhang
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Xinheng Zhang
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Guanming Shao
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Guanming Shao
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Yangyang Shao
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Yangyang Shao
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Zezhong Hu
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zezhong Hu
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Keyu Feng
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Keyu Feng
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Keyu Feng
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zi Xie
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zi Xie
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Hongxin Li
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Hongxin Li
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Hongxin Li
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Weiguo Chen
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Weiguo Chen
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Weiguo Chen
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Wencheng Lin
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Wencheng Lin
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Wencheng Lin
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- Hengxing Yuan
- State Key Laboratory of Microbial Technology, Helmholtz International Lab for Anti-infectives, Institute of Microbial Technology, Shandong University–Helmholtz Institute of Biotechnology, Shandong University, Qingdao, China
- Hailong Wang
- State Key Laboratory of Microbial Technology, Helmholtz International Lab for Anti-infectives, Institute of Microbial Technology, Shandong University–Helmholtz Institute of Biotechnology, Shandong University, Qingdao, China
- Jun Fu
- State Key Laboratory of Microbial Technology, Helmholtz International Lab for Anti-infectives, Institute of Microbial Technology, Shandong University–Helmholtz Institute of Biotechnology, Shandong University, Qingdao, China
- Qingmei Xie
- Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qingmei Xie
- Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou, China
- Qingmei Xie
- Key Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2022.832255
- Journal volume & issue
-
Vol. 9
Abstract
African swine fever (ASF) is a highly contact infectious disease caused by the African swine fever virus (ASFV). The extremely complex structure and infection mechanism make it difficult to control the spread of ASFV and develop the vaccine. The ASFV genome is huge with many antigenic genes. Among them, CP204L (p30), CP530R (pp62), E183L (p54), B646L (p72), and EP402R (CD2v) are involved in the process of the virus cycle, with strong immunogenicity and the ability to induce the body to produce neutralizing antibodies. In this study, the recombinant virus rBartha-K61-pASFV that expresses the above ASFV antigen genes was constructed by Red/ET recombineering technology using pseudorabies virus (PRV) vaccine strain Bartha-K61. Western blot analysis showed that the ASFV antigen gene was expressed and the recombinant virus showed good genetic stability and proliferation characteristics in 15 continuous generations on porcine kidney (PK15) cells. The results of immunoassay of piglets and mice showed that rBartha-K61-pASFV had good immunogenicity and could induce higher antibody levels in the body. Therefore, PRV was a promising viral vector for expressing the ASFV antigen gene, and all the experiments in this study laid a foundation for the further development of a new viral vector vaccine of ASFV.
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