The expression of the hormone estrogen in the gonads of green Asian green mussel (Perna viridis) as biomarker of estrogenic compound on the Banyu Urip s and Kenjeran Waters, East Java

Abstract

Asian Green Mussel is one the ideal bivalves to investigate the estrogenic contamination because of its life that is sedentary, attached, and sensitive to environmental changes. Estrogenic compound is very harmful to organisms because it can resemble the estrogen hormone (xenoestrogen) and it has high accumulation power in the body. Xenoestrogen can bind to ER expressed in the E2 form. The research method used in this research was ELISA kit. Based on the research result, the ER in the body of Asian Green Mussel in Banyu Urip is 0.125 ng/l in male and 8.2 ng/l in the female. In Kenjeran waters, Perna Viridis is 5.8 ng/l in male and 2.9 ng/l in the female, the estradiol concentrations in water and body of male and female of Asian Green Mussel in Banyu Urip are 4 ng/l; 0.25 ng/l and 30.6 ng/l, respectively. Meanwhile, in Kenjeran are 80 ng/l; 39.9 ng/l and 5.9 ng/l, respectively. E2 Gonads of male Asian Green Mussel in Banyu Urip tend to be lower than in Kenjeran. This is due to the presence of estrogenic compounds that will disrupt the hormone system within the Asian Green Mussel’s body. Thus the Asian Green Mussel synthesizes the estrogen hormone more than the usual level. If this condition continues, it can change the Asian Green Mussel that is supposed to be male changes in female due to excessive synthesis of estrogen hormone. This change may cause an unbalanced condition that will affect the population within an aquatic ecosystem.

Keywords

• Estrogenic compund
• perna viridis
• biomarker
• hormones