Human-Derived Fibrin Glue: In Vitro Antibacterial Effects and Antibiotic Permeation

Abstract

Objectives: This study investigated the in vitro antibacterial efficacy and antibiotic permeation of human-derived fibrin glue (FG). Materials and Methods: FG was prepared under sterile conditions by the Blood Bank of Gazi University Faculty of Medicine. In this study, cultivations were performed in 5 main groups: Group 1 (control) - only FG; Group 2a (control) - pure Staphylococcus aureus (SA) and Group 2b (control) - pure Staphylococcus epidermidis (SE); Group 3a (control) - SA+antibiotic and Group 3b (control) - SE+antibiotic; Group 4a - FG+SA and Group 4b - FG+SE; Group 5a - FG+SA+antibiotic and Group 5b - FG+SA+antibiotic. Results: Group 1 showed no bacterial growth, whereas Group 2a and Group 2b, Group 4a and Group 4b showed bacterial growth. Group 5a and Group 5b showed no growth. Conclusion: Although FG has no antibacterial efficacy in vitro, it may be used safely due to antibiotic permeation in diseases with either infected or non-infected ocular surface that require suturing. (Turk J Ophthalmol 2014; 44: 347-50)

Keywords

• Antibacterial activity
• corneal perforation
• fibrin glue
• sutureless amniotic membrane transplantation