Mapping cortical mesoscopic networks of single spiking cortical or sub-cortical neurons
Dongsheng Xiao,
Matthieu P Vanni,
Catalin C Mitelut,
Allen W Chan,
Jeffrey M LeDue,
Yicheng Xie,
Andrew CN Chen,
Nicholas V Swindale,
Timothy H Murphy
Affiliations
Dongsheng Xiao
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada
Matthieu P Vanni
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada
Catalin C Mitelut
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada
Allen W Chan
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada
Jeffrey M LeDue
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada
Yicheng Xie
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada
Andrew CN Chen
Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China
Nicholas V Swindale
Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, Canada
Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada
Understanding the basis of brain function requires knowledge of cortical operations over wide-spatial scales, but also within the context of single neurons. In vivo, wide-field GCaMP imaging and sub-cortical/cortical cellular electrophysiology were used in mice to investigate relationships between spontaneous single neuron spiking and mesoscopic cortical activity. We make use of a rich set of cortical activity motifs that are present in spontaneous activity in anesthetized and awake animals. A mesoscale spike-triggered averaging procedure allowed the identification of motifs that are preferentially linked to individual spiking neurons by employing genetically targeted indicators of neuronal activity. Thalamic neurons predicted and reported specific cycles of wide-scale cortical inhibition/excitation. In contrast, spike-triggered maps derived from single cortical neurons yielded spatio-temporal maps expected for regional cortical consensus function. This approach can define network relationships between any point source of neuronal spiking and mesoscale cortical maps.
