3D single-molecule super-resolution microscopy with a tilted light sheet

Anna-Karin Gustavsson; Petar N. Petrov; Maurice Y. Lee; Yoav Shechtman; W. E. Moerner

doi:10.1038/s41467-017-02563-4

Nature Communications (Jan 2018)

3D single-molecule super-resolution microscopy with a tilted light sheet

Anna-Karin Gustavsson,
Petar N. Petrov,
Maurice Y. Lee,
Yoav Shechtman,
W. E. Moerner

Affiliations

Anna-Karin Gustavsson: Department of Chemistry, Stanford University
Petar N. Petrov: Department of Chemistry, Stanford University
Maurice Y. Lee: Department of Chemistry, Stanford University
Yoav Shechtman: Department of Chemistry, Stanford University
W. E. Moerner: Department of Chemistry, Stanford University

DOI: https://doi.org/10.1038/s41467-017-02563-4
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

Read online

Light-sheet single-molecule 3D super-resolution microscopes can’t image close to a coverslip or may require complex apparatus. Here the authors overcome such limitations using a tilted light sheet strategy with long axial range point spread functions on a standard inverted microscope.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal