Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions

Stéphanie Miserey-Lenkei; Hugo Bousquet; Olena Pylypenko; Sabine Bardin; Ariane Dimitrov; Gaëlle Bressanelli; Raja Bonifay; Vincent Fraisier; Catherine Guillou; Cécile Bougeret; Anne Houdusse; Arnaud Echard; Bruno Goud

doi:10.1038/s41467-017-01266-0

Nature Communications (Nov 2017)

Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions

Stéphanie Miserey-Lenkei,
Hugo Bousquet,
Olena Pylypenko,
Sabine Bardin,
Ariane Dimitrov,
Gaëlle Bressanelli,
Raja Bonifay,
Vincent Fraisier,
Catherine Guillou,
Cécile Bougeret,
Anne Houdusse,
Arnaud Echard,
Bruno Goud

Affiliations

Stéphanie Miserey-Lenkei: Institut Curie, PSL Research University, CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport
Hugo Bousquet: Institut Curie, PSL Research University, CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport
Olena Pylypenko: Institut Curie, PSL Research University, CNRS, UMR 144, Structural Motility
Sabine Bardin: Institut Curie, PSL Research University, CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport
Ariane Dimitrov: Institut Curie, PSL Research University, CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport
Gaëlle Bressanelli: Institut Curie, PSL Research University, CNRS, UMR 144, Structural Motility
Raja Bonifay: Institut Curie, PSL Research University, CNRS, UMR 144, Structural Motility
Vincent Fraisier: Institut Curie, PSL Research University, CNRS, UMR 144, Cell and Tissue Imaging Facility (PICT-IBiSA)
Catherine Guillou: ICSN-CNRS
Cécile Bougeret: Biokinesis
Anne Houdusse: Institut Curie, PSL Research University, CNRS, UMR 144, Structural Motility
Arnaud Echard: Institut Pasteur, CNRS UMR3691, Membrane Traffic and Cell Division
Bruno Goud: Institut Curie, PSL Research University, CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport

DOI: https://doi.org/10.1038/s41467-017-01266-0
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 13

Abstract

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Actin and microtubules play important roles in Golgi structure and function but how they are connected is poorly understood. Here the authors show that KIF20A is involved in the fission process and, in association with Myosin II, serves to anchor RAB6 on Golgi/TGN membranes near microtubules nucleating sites.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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