Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)
Raquel D. C. C. Bandeira,
Thais M. Uekane,
Carolina P. da Cunha,
Luths R. O. Geaquinto,
Valnei S. Cunha,
Janaina M. R. Caixeiro,
Ronoel Luiz O. Godoy,
Marcus Henrique C. de la Cruz
Affiliations
Raquel D. C. C. Bandeira
Universidade Federal Rural do Rio de Janeiro
Thais M. Uekane
Instituto Nacional de Metrologia, Qualidade e Tecnologia
Carolina P. da Cunha
Instituto Nacional de Metrologia, Qualidade e Tecnologia
Luths R. O. Geaquinto
Instituto Nacional de Metrologia, Qualidade e Tecnologia
Valnei S. Cunha
Instituto Nacional de Metrologia, Qualidade e Tecnologia
Janaina M. R. Caixeiro
Instituto Nacional de Metrologia, Qualidade e Tecnologia
A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.
