AMB Express
(Mar 2023)
Improving ethanol tolerance of ethyl carbamate hydrolase by diphasic high pressure molecular dynamic simulations
Qijia Zan,
Mengfei Long,
Nan Zheng,
Zehua Zhang,
Huimin Zhou,
Xinjie Xu,
Tolbert Osire,
Xiaole Xia
Affiliations
Qijia Zan
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
Mengfei Long
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
Nan Zheng
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
Zehua Zhang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
Huimin Zhou
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
Xinjie Xu
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
Tolbert Osire
Faculty of Biology, Shenzhen MSU-BIT University
Xiaole Xia
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University
DOI
https://doi.org/10.1186/s13568-023-01538-7
Journal volume & issue
Vol. 13,
no. 1
Abstract
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Key points 1 Diphasic high pressure molecular dynamic simulations were applied for ration engineering of ethyl carbamate hydrolase with improved ethanol tolerance. 2 The specific activity of H68A/K70R/S325N was 3.42-fold higher than WT. 3 The tolerance of triple mutant H68A/K70R/S325N to 20%(v/v) ethanol was increased to 41.16%, which was 5.02-fold higher than WT.
Keywords
Published in AMB Express
ISSN
2191-0855 (Online)
Publisher
SpringerOpen
Country of publisher
United Kingdom
LCC subjects
Technology: Chemical technology: Biotechnology
Science: Microbiology
Website
http://www.amb-express.com/
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