PRP significantly promotes the adhesion and migration of vascular smooth muscle cells on stent material

Abstract

Abstract Background The adhesion and survival state of cells on scaffold material is a major problem in tissue-engineered blood vessel (TEBV) culture. Platelet-rich plasma (PRP) contains a large amount of biologically active factors and fibrin, which is expected to play an important role in TEBV culture. Purpose To combine PRP with cells and scaffold material to promote cell adhesion and biological activity on the scaffold material. Methods The adhesion status and migration of SMCs under the optimal concentration suitable for SMC growth and the optimal concentration of PRP were examined by scanning electron microscopy, HE staining, CCK-8 assays, qPCR, WB, and other experimental methods and compared with those under the conventional culture (20% FBS); finally, the effect of PRP on the deposition of ECM in vascular tissue engineering culture was verified by three-dimensional culture. Results PRP at 20% is a suitable concentration for SMCs. Compared with the control group, the 20% PRP group had better migration, and the number of SMC adhesions was significantly higher than that of the control group. In addition, collagen deposition in the experimental group was significantly higher than that in the control group. Conclusion PRP (20%) can promote SMC adhesion, migration, and collagen deposition on the scaffold material.

Keywords

• Platelet-rich plasma (PRP)
• Smooth muscle cell (SMC)
• Cell migration assays
• Decellularized extracellular matrix (ECM)