Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast
Noah Dephoure,
Sunyoung Hwang,
Ciara O'Sullivan,
Stacie E Dodgson,
Steven P Gygi,
Angelika Amon,
Eduardo M Torres
Affiliations
Noah Dephoure
Department of Cell Biology, Harvard Medical School, Boston, United States
Sunyoung Hwang
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, United States
Ciara O'Sullivan
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, United States
Stacie E Dodgson
David H. Koch Institute for Integrative Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States
Steven P Gygi
Department of Cell Biology, Harvard Medical School, Boston, United States
Angelika Amon
David H. Koch Institute for Integrative Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States
Eduardo M Torres
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, United States
Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis. Indeed aneuploid cells harbor increased levels of reactive oxygen species (ROS). Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains. Our results show that aneuploidy causes alterations in metabolism and redox homeostasis. Cells respond to these alterations through both transcriptional and posttranscriptional mechanisms.
