Study of the Wobenzym Enzyme Preparation Effect on the Formation of Bacterial Biofilms

Abstract

Background. According to the International Center for Disease Control (CDC), 65–80% of all bacterial infections recorded in countries around the world are associated with the ability of their pathogens to form biofilms. To eliminate biofilms, various methods are being developed, including those involving the use of enzymes, proteins, plant extracts, and composite antibacterial coatings. Both antibiotic-resistant and sensitive strains have the ability to form biofilms, which confirms the relevance of the problem of biofilm formation by bacterial cells and the necessity of finding a solution to the treatment of infections caused by film-forming isolates.The aim was to study the influence of the Wobenzym enzyme preparation on the formation of biofilms of clinical bacterial isolates and to determine the presence of a potentiating effect on the action of antibiotics.Material and methods. A bacteriological method was used in the study. In this work, 20 strains that differed in their ability to form films were studied.Results. The most pronounced film-forming ability was exhibited by Escherichia coli (OD=1.0) and Enterococcus faecalis (OD=0.649), isolated from the discharge of the cervical canal, as well as Enterobacter aerogenes (OD=0.406), isolated from the discharge of the pharynx of a newborn child. Cultivation of all studied strains in the presence of Wobenzym significantly reduces their film-forming ability (OD without the addition of enzyme — 0.255±0.005; with enzyme — 0.084±0.006, P=0.0009). Potentiation of the antibiotics ampicillin and amikacin by the Wobenzym enzyme preparation was confirmed by a more than two-fold decrease in CFU/µl.Conclusion. Cultivation of Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and Enterobacter aerogenes strains in the presence of Wobenzym significantly reduces their ability to form biofilms, which can be used to prevent biofilm formation and eradicate strains of opportunistic microorganisms that cause infectious and inflammatory processes in normally non-sterile mucous membrane loci of the human body. The combined use of Wobenzym with antibacterial therapy has a direct potentiating effect of antibacterial drugs, as well as an indirect effect that increases the clinical effectiveness of antibacterial therapy by reducing the film-forming ability.

Keywords

• wobenzym
• e. coli, biofilms, film formation, enzymes