Frontiers in Genetics (Feb 2021)
Global Transcriptomic Analyses Reveal Genes Involved in Conceptus Development During the Implantation Stages in Pigs
- Xupeng Zang,
- Xupeng Zang,
- Xupeng Zang,
- Ting Gu,
- Ting Gu,
- Ting Gu,
- Qun Hu,
- Qun Hu,
- Qun Hu,
- Zhiqian Xu,
- Zhiqian Xu,
- Zhiqian Xu,
- Yanshe Xie,
- Yanshe Xie,
- Yanshe Xie,
- Chen Zhou,
- Chen Zhou,
- Chen Zhou,
- Enqin Zheng,
- Enqin Zheng,
- Enqin Zheng,
- Sixiu Huang,
- Sixiu Huang,
- Sixiu Huang,
- Zheng Xu,
- Zheng Xu,
- Zheng Xu,
- Fanming Meng,
- Gengyuan Cai,
- Gengyuan Cai,
- Gengyuan Cai,
- Zhenfang Wu,
- Zhenfang Wu,
- Zhenfang Wu,
- Linjun Hong,
- Linjun Hong,
- Linjun Hong
Affiliations
- Xupeng Zang
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xupeng Zang
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Xupeng Zang
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Ting Gu
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Ting Gu
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Ting Gu
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Qun Hu
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qun Hu
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Qun Hu
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Zhiqian Xu
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zhiqian Xu
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zhiqian Xu
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Yanshe Xie
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Yanshe Xie
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Yanshe Xie
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Chen Zhou
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Chen Zhou
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Chen Zhou
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Enqin Zheng
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Enqin Zheng
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Enqin Zheng
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Sixiu Huang
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Sixiu Huang
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Sixiu Huang
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Zheng Xu
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zheng Xu
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zheng Xu
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Fanming Meng
- Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China
- Gengyuan Cai
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Gengyuan Cai
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Gengyuan Cai
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Zhenfang Wu
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zhenfang Wu
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Zhenfang Wu
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- Linjun Hong
- National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, China
- Linjun Hong
- Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, China
- Linjun Hong
- Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, China
- DOI
- https://doi.org/10.3389/fgene.2021.584995
- Journal volume & issue
-
Vol. 12
Abstract
Prenatal mortality remains a significant concern to the pig farming industry around the world. Spontaneous fetal loss ranging from 20 to 45% by term occur after fertilization, with most of the loss happening during the implantation period. Since the factors regulating the high mortality rates of early conceptus during implantation phases are poorly understood, we sought to analyze the overall gene expression changes during this period, and identify the molecular mechanisms involved in conceptus development. This work employed Illumina’s next-generation sequencing (RNA-Seq) and quantitative real-time PCR to analyze differentially expressed genes (DEGs). Soft clustering was subsequently used for the cluster analysis of gene expression. We identified 8236 DEGs in porcine conceptus at day 9, 12, and 15 of pregnancy. Annotation analysis of these genes revealed rRNA processing (GO:0006364), cell adhesion (GO:1904874), and heart development (GO:0007507), as the most significantly enriched biological processes at day 9, 12, and 15 of pregnancy, respectively. In addition, we found various genes, such as T-complex 1, RuvB-like AAA ATPase 2, connective tissue growth factor, integrins, interferon gamma, SLA-1, chemokine ligand 9, PAG-2, transforming growth factor beta receptor 1, and Annexin A2, that play essential roles in conceptus morphological development and implantation in pigs. Furthermore, we investigated the function of PAG-2 in vitro and found that PAG-2 can inhibit trophoblast cell proliferation and migration. Our analysis provides a valuable resource for understanding the mechanisms of conceptus development and implantation in pigs.
Keywords
