OncoTargets and Therapy (Dec 2018)
MiR-411 suppresses the development of bladder cancer by regulating ZnT1
Abstract
Yang Liu, Tao Liu, Hongwei Jin, Lei Yin, Hongyuan Yu, Jianbin Bi Urology Surgery, First Affiliated Hospital of China Medical University, Shenyang, China Background: At present, the molecular genetics of the development and progression of bladder cancer are still unclear. In recent years, the pathological relevance and significance of microRNAs (miRNAs) in bladder cancer have attracted increasing attention.Methods: The expressions of miR-411 and zinc transporter 1 (ZnT1) in bladder cancer were determined by western blot and real-time PCR. Biological software, luciferase reporter gene, Western blot and real-time PCR were used to determine the regulatory effect of miR-411 on ZnT1. MTT and transwell were used to confirm the regulatory effect of miR-411 on bladder cancer cells. MTT and transwell were used to find how miR-411 modulated the biological activity of bladder cancer cells by regulating ZnT1. Results: The expression of miR-411 was low in bladder cancer and was negatively correlated with ZnT1. MiR-411 can inhibit the activity and the expression of ZnT1. MiR-411 can inhibit the growth and metastasis of bladder cancer cells. MiR-411 inhibited the growth and metastasis of bladder cancer cells by targeting ZnT1. Conclusion: The miR-411 target ZnT1 may provide a potential therapeutic target for the treatment of bladder cancer. Keywords: miR-411, ZnT1, bladder cancer, proliferation, metastasis
