Investigation of tumor tissue alteration after experimental chemotherapy

Abstract

The aim of the present work was the study of tumor tissue alteration after new antineoplastic substance administration in comparison with widely investigated 5-fluorouracil. White rats bearing Plisse lymphosarcoma, sarcoma 45, Walker carcino-sarcoma, sarcoma 180 were used in the study. Pieces of tumor tissue were fixed in 10% neutral formaldehyde and processed using standard procedure. Histological slides were stained by hematoxylin and eosin method, van Gieson, Mc Manus, Feulgen and Picro-Mallori methods. Apoptotic markers p-53 and bcl-2 were detected using immunohistological method. Apoptotic index, mitotic index, comparative area of necrotic tissue estimation have been carried out. The unidirectional changes of experimental tumor tissue, the significant tumor growth inhibition, alteration, scar tissue formation, blood circulation disturbances due to FP-8 and 5-fluorouracil administration were shown.

Keywords

• fluoropyrimidines
• histology
• apoptosis
• necrosis
• mitosis