Investigation of Polyacrylic Acid Toxicity in Human Breast Cancer (MCF-7) and Mouse Fibroblast (L-929) Cell Lines

Abstract

In recent years, biopolymers have been widely used in various fields of medicine. Before using any polymer, its biocompatibility should be examined. Polyacrylic acid (PAA), a polyelectrolyte, is known to be used as an adjuvant effect in immunology, anti-thrombogenic effect in medical experiments, and as a carrier in drug delivery systems. Although there are studies on various conjugates and nanoparticles of PAA, studies on its toxicity alone are limited. Determination of toxicity in biopolymer studies is extremely important. Cultures of various cells are used for toxicity analyses. This study aimed to investigate the toxicity of PAA in human breast cancer (MCF-7) and mouse fibroblast (L-929) cell lines by various methods. Cell culture, 3-(4,5-dimethyltriazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue and 4,6-diamidino 2 phenylindole (DAPI) methods were used in the study. The half-maximal effective concentration (EC50) value of PAA was 6.6 mg/ml in MCF 7 cells and 1.8 mg/ ml in L-929 cells. Apoptosis was observed in cells on the increasing PAA concentration with DAPI. With these results, the cytotoxic properties of PAA were determined in vitro. Accordingly, the biocompatibility of polymers to be used in modeling should be supported by in vitro and in vivo studies.

Keywords

• apoptosis
• biocompatibility
• cytotoxicity
• polyacrylic acid