Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana

Abstract

In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types [1]. Nuclear RNA was neglected for a long time as not being representative for transcriptomic studies. In recent years, however, there have been reports describing the validity of nuclear RNA for these types of studies [2,3]. Here we describe the generation, quality assessment and analysis of nuclear transcriptomic data from Arabidopsis embryos published by Slane et al. (2014) [4]. Comparison of nuclear with cellular gene expression demonstrated the usefulness of nuclear transcriptomics.

Keywords

• Arabidopsis thaliana
• Gene expression
• Microarray
• Nuclear and cellular transcriptome
• Pro-embryo and suspensor transcriptome