Nature Communications (Sep 2020)
DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe
- Qi Liu,
- Qing-tao He,
- Xiaoxuan Lyu,
- Fan Yang,
- Zhong-liang Zhu,
- Peng Xiao,
- Zhao Yang,
- Feng Zhang,
- Zhao-ya Yang,
- Xiao-yan Wang,
- Peng Sun,
- Qian-wen Wang,
- Chang-xiu Qu,
- Zheng Gong,
- Jing-yu Lin,
- Zhen Xu,
- Shao-le Song,
- Shen-ming Huang,
- Sheng-chao Guo,
- Ming-jie Han,
- Kong-kai Zhu,
- Xin Chen,
- Alem W. Kahsai,
- Kun-Hong Xiao,
- Wei Kong,
- Fa-hui Li,
- Ke Ruan,
- Zi-jian Li,
- Xiao Yu,
- Xiao-gang Niu,
- Chang-wen Jin,
- Jiangyun Wang,
- Jin-peng Sun
Affiliations
- Qi Liu
- Institute of Biophysics, Chinese Academy of Sciences
- Qing-tao He
- Institute of Biophysics, Chinese Academy of Sciences
- Xiaoxuan Lyu
- Institute of Biophysics, Chinese Academy of Sciences
- Fan Yang
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Physiology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Zhong-liang Zhu
- School of Life Sciences, University of Science and Technology of China
- Peng Xiao
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Zhao Yang
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Physiology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Feng Zhang
- Institute of Biophysics, Chinese Academy of Sciences
- Zhao-ya Yang
- Institute of Biophysics, Chinese Academy of Sciences
- Xiao-yan Wang
- Institute of Biophysics, Chinese Academy of Sciences
- Peng Sun
- Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences
- Qian-wen Wang
- Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences
- Chang-xiu Qu
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Zheng Gong
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Jing-yu Lin
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Physiology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Zhen Xu
- Institute of Biophysics, Chinese Academy of Sciences
- Shao-le Song
- Institute of Biophysics, Chinese Academy of Sciences
- Shen-ming Huang
- Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University
- Sheng-chao Guo
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Ming-jie Han
- Institute of Biophysics, Chinese Academy of Sciences
- Kong-kai Zhu
- School of Biological Science and Technology, University of Jinan
- Xin Chen
- Department of Medicinal Chemistry, School of Pharmaceutical Engineering and Life Science, Changzhou University
- Alem W. Kahsai
- Duke University, School of Medicine
- Kun-Hong Xiao
- Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh
- Wei Kong
- Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University
- Fa-hui Li
- Institute of Biophysics, Chinese Academy of Sciences
- Ke Ruan
- Hefei National Laboratory for Physical Science at the Microscale, University of Science and Technology of China
- Zi-jian Li
- Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University
- Xiao Yu
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Physiology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- Xiao-gang Niu
- Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, School of Life Sciences, Peking University
- Chang-wen Jin
- Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, School of Life Sciences, Peking University
- Jiangyun Wang
- Institute of Biophysics, Chinese Academy of Sciences
- Jin-peng Sun
- Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo college of Medicine, Shandong University
- DOI
- https://doi.org/10.1038/s41467-020-18433-5
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 17
Abstract
Characterization of dynamic conformational changes in membrane protein complexes by NMR spectroscopy remains challenging. Here authors report the site-specific incorporation of 4-trimethylsilyl phenylalanine (TMSiPhe) into proteins, which enabled the characterization of multiple conformational states of a phospho-β2 adrenergic receptor/β-arrestin-1 complex in response to different receptor ligands.
