Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]

Abstract

Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic modification of RNA. These methods have different benefits and limitations. This review aims to outline the current state-of-the-art techniques and point out their benefits and limitations.

Keywords

• Chemical Biology of the Cell
• Control of Gene Expression
• Genomics