Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method
Ekaterina D. Mordvinova,
Polina A. Nikitina,
Olga I. Yarovaya,
Ekaterina A. Volosnikova,
Denis E. Murashkin,
Anastasiya A. Isaeva,
Tatiana Y. Koldaeva,
Valery P. Perevalov,
Nariman F. Salakhutdinov,
Dmitriy N. Shcherbakov
Affiliations
Ekaterina D. Mordvinova
Department of Medicinal Chemistry, N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentyev ave., 9, 630090 Novosibirsk, Russia
Polina A. Nikitina
Department of Fine Organic Synthesis and Chemistry of Dyes, D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya sq., 9, 125047 Moscow, Russia
Olga I. Yarovaya
Department of Medicinal Chemistry, N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentyev ave., 9, 630090 Novosibirsk, Russia
Ekaterina A. Volosnikova
State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
Denis E. Murashkin
State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
Anastasiya A. Isaeva
State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
Tatiana Y. Koldaeva
Department of Fine Organic Synthesis and Chemistry of Dyes, D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya sq., 9, 125047 Moscow, Russia
Valery P. Perevalov
Department of Fine Organic Synthesis and Chemistry of Dyes, D.I. Mendeleev University of Chemical Technology of Russia, Miusskaya sq., 9, 125047 Moscow, Russia
Nariman F. Salakhutdinov
Department of Medicinal Chemistry, N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Lavrentyev ave., 9, 630090 Novosibirsk, Russia
Dmitriy N. Shcherbakov
State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, Russia
Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, plays a crucial role in the pathogenesis of COVID-19. ACE2 targeting holds the promise for preventing and inhibiting SARS-CoV-2 infection. In this work, we describe the development and use of a test system based on competitive ELISA for the primary screening of potential antiviral compounds. We studied the activity of the library of dyes of different groups. Several dyes (ortho-cresolphthalein, eosin (free acid), eosin (Na salt)) that inhibited the interaction of ACE2 with the spike proteins of SARS-CoV-2 have been identified among the candidates. A potential antiviral drug, methylene blue, did not show activity in our study. We believe that our results can help in the further search for inhibitors of interaction between the coronavirus spike protein and ACE2 receptor.
