Nature Communications (May 2021)

Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling

  • Fu-Lung Yeh,
  • Shang-Lin Chang,
  • Golam Rizvee Ahmed,
  • Hsin-I Liu,
  • Luh Tung,
  • Chung-Shu Yeh,
  • Leah Stands Lanier,
  • Corina Maeder,
  • Che-Min Lin,
  • Shu-Chun Tsai,
  • Wan-Yi Hsiao,
  • Wei-Hau Chang,
  • Tien-Hsien Chang

DOI
https://doi.org/10.1038/s41467-021-23459-4
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 9

Abstract

Read online

Yeast helicase Prp28 promotes the first step of spliceosome remodeling. By placing a photoactivatable unnatural amino acid in Prp28, the authors capture Prp28 in action revealing its dynamic interactions and cofactor Npl3.