Nature Communications (Oct 2020)
Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae
- David Vizarraga,
- Akihiro Kawamoto,
- U. Matsumoto,
- Ramiro Illanes,
- Rosa Pérez-Luque,
- Jesús Martín,
- Rocco Mazzolini,
- Paula Bierge,
- Oscar Q. Pich,
- Mateu Espasa,
- Isabel Sanfeliu,
- Juliana Esperalba,
- Miguel Fernández-Huerta,
- Margot P. Scheffer,
- Jaume Pinyol,
- Achilleas S. Frangakis,
- Maria Lluch-Senar,
- Shigetarou Mori,
- Keigo Shibayama,
- Tsuyoshi Kenri,
- Takayuki Kato,
- Keiichi Namba,
- Ignacio Fita,
- Makoto Miyata,
- David Aparicio
Affiliations
- David Vizarraga
- Instituto de Biología Molecular de Barcelona (IBMB-CSIC)
- Akihiro Kawamoto
- Graduate School of Frontier Biosciences, Osaka University
- U. Matsumoto
- Graduate School of Science, Osaka City University
- Ramiro Illanes
- Instituto de Biología Molecular de Barcelona (IBMB-CSIC)
- Rosa Pérez-Luque
- Instituto de Biología Molecular de Barcelona (IBMB-CSIC)
- Jesús Martín
- Instituto de Biología Molecular de Barcelona (IBMB-CSIC)
- Rocco Mazzolini
- EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
- Paula Bierge
- Laboratori de Recerca en Microbiologia i Malalties Infeccioses, Institut d’Investigació i Innovació Parc Taulí (I3PT), Hospital Universitari Parc Taulí, Universitat Autònoma de Barcelona
- Oscar Q. Pich
- Laboratori de Recerca en Microbiologia i Malalties Infeccioses, Institut d’Investigació i Innovació Parc Taulí (I3PT), Hospital Universitari Parc Taulí, Universitat Autònoma de Barcelona
- Mateu Espasa
- Departament de Microbiologia, Hospital Universitari Parc Taulí, Universitat Autònoma de Barcelona
- Isabel Sanfeliu
- Departament de Microbiologia, Hospital Universitari Parc Taulí, Universitat Autònoma de Barcelona
- Juliana Esperalba
- Departament de Microbiologia, Hospital Universitari Vall d´Hebron, Universitat Autònoma de Barcelona
- Miguel Fernández-Huerta
- Departament de Microbiologia, Hospital Universitari Vall d´Hebron, Universitat Autònoma de Barcelona
- Margot P. Scheffer
- Buchmann Institute for Molecular Life Sciences
- Jaume Pinyol
- Departament de Bioquímica i Biologia Molecular, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona
- Achilleas S. Frangakis
- Buchmann Institute for Molecular Life Sciences
- Maria Lluch-Senar
- EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
- Shigetarou Mori
- Department of Bacteriology II, National Institute of Infectious Diseases
- Keigo Shibayama
- Department of Bacteriology II, National Institute of Infectious Diseases
- Tsuyoshi Kenri
- Department of Bacteriology II, National Institute of Infectious Diseases
- Takayuki Kato
- Graduate School of Frontier Biosciences, Osaka University
- Keiichi Namba
- Graduate School of Frontier Biosciences, Osaka University
- Ignacio Fita
- Instituto de Biología Molecular de Barcelona (IBMB-CSIC)
- Makoto Miyata
- Graduate School of Science, Osaka City University
- David Aparicio
- Instituto de Biología Molecular de Barcelona (IBMB-CSIC)
- DOI
- https://doi.org/10.1038/s41467-020-18777-y
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 16
Abstract
Adhesion of the human pathogen Mycoplasma pneumoniae to pulmonary epithelial cells is mediated by a transmembrane complex composed of proteins P1 and P40/P90. Here, the authors present the structures of M. pneumoniae P1 and P40/P90, show that P40/P90 binds sialylated oligosaccharides and have also determined the crystal structures of P40/P90 complexes with 3’-Sialyllactose and 6’-Sialyllactose, which provide insights into the mechanisms of adhesion and gliding on host cell surfaces.
