An expedient and rapid High-Performance Liquid Chromatographic method for the kinetic study of Ketoprofen

Abstract

A convenient and rapid High-Performance Liquid Chromatography (HPLC) based protocol was optimized for the routine analysis and kinetic study of Ketoprofen (KP) in plasma and urinary execration of patients. The optimized HPLC method was found to be linear over the wide range of KP concentration (1–90 µg/mL) with substantial recovery rates (95.5–97.63%) of calibration standards at an improved detection (0.02 μg/mL LoD) and quantitation limit (0.06 μg/mL LoQ) and acceptable inter and intraday variability (RSD ≤ 7.75%). The maximum amount (μg/mL) of KP was excreted through the urine of all participated patients during the first two hours (h) of oral administration. Likewise, a fast depletion in blood plasma concentration started early after oral administration. Overall, the present method provided a convenient, rapid, and reliable solution for the routine analysis and kinetic study of KP in biological samples. Keywords: HPLC, Ketoprofen, Rapid analysis, Urinary and plasma excretion