Nature Communications (Sep 2020)

Characterization and mitigation of gene expression burden in mammalian cells

  • Timothy Frei,
  • Federica Cella,
  • Fabiana Tedeschi,
  • Joaquín Gutiérrez,
  • Guy-Bart Stan,
  • Mustafa Khammash,
  • Velia Siciliano

DOI
https://doi.org/10.1038/s41467-020-18392-x
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 14

Abstract

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The design of genetic networks in mammalian cells is still slow and often fails. Here the authors show that miRNA-based incoherent feedforward loop circuits can be used to alleviate cellular burden.