Diagnostics (Aug 2022)

Reproducibility and Reliability of Spectralis II OCT Angiography Vascular Measurements

  • Marco R. Pastore,
  • Alberto Grotto,
  • Francesca Vezzoni,
  • Andrea Gaggino,
  • Serena Milan,
  • Stefano Gouigoux,
  • Pier Luigi Guerin,
  • Alex L. Vinciguerra,
  • Gabriella Cirigliano,
  • Daniele Tognetto

DOI
https://doi.org/10.3390/diagnostics12081908
Journal volume & issue
Vol. 12, no. 8
p. 1908

Abstract

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Purpose: to investigate the reproducibility and reliability of OCT-A vascular measurements using Heidelberg Spectralis II OCT-A. Methods: a prospective study involving a single eye of patients aged 18 or older with no ocular disease. In order to investigate the reliability of the first and second OCT-A scans, the coefficient of variation of the foveal avascular zone (FAZ) and the vessel density (VD) in the superficial (SCP), intermediate (ICP) and deep capillary plexus (DCP) were calculated. Results: A total of 75 eyes were included in the study. The mean FAZ in the first and second scan was 0.36 × 0.13 mm2 and 0.37 × 0.12 mm2, respectively, in the SCP, 0.23 × 0.10 mm2 and 0.23 × 0.09 mm2 in the ICP, and 0.42 × 0.11 mm2 and 0.43 × 0.12 mm2 in the DCP. The overall VD was 36.05 × 9.01 and 35.33 × 9.92 at the first and second scan, respectively, in the SCP, 21.87 × 5.00 and 21.32 × 5.56 in the ICP, and 23.84 × 6.53 and 23.20 × 6.83 in the DCP. No statistically significant differences in FAZ measurements and VD in all sectors of each capillary plexus were observed between the first and second scan (p > 0.05). Conclusion: our study demonstrated the good reproducibility and reliability of OCT-A vascular measurements in the analysis of the FAZ and the quantification of VD in each capillary plexus of the retina.

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