Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report

Abstract

Xenogenic mammalian sperm heads injected into mouse ovulated oocytes decondense and form pronuclei in which sperm DNA parameters can be evaluated. We suggest that this approach can be used for the assessment of sperm DNA damage level and the evaluation of how certain sperm treatments (freezing, lyophilization, etc.) influence the quality of spermatozoa.

Keywords

• sperm
• oocyte
• DNA damage