Anthraquinones from the Aerial Parts of <i>Rubia cordifolia</i> with Their NO Inhibitory and Antibacterial Activities
Han Luo,
Wei Qin,
Hong Zhang,
Fu-Cai Ren,
Wen-Tao Fang,
Qing-Hua Kong,
Liu Yang,
Jian-Mei Zhang,
Cheng-Wu Fang,
Jiang-Miao Hu,
Shou-Jin Liu
Affiliations
Han Luo
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230011, China
Wei Qin
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230011, China
Hong Zhang
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230011, China
Fu-Cai Ren
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Wen-Tao Fang
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230011, China
Qing-Hua Kong
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Liu Yang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Jian-Mei Zhang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Cheng-Wu Fang
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230011, China
Jiang-Miao Hu
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
Shou-Jin Liu
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230011, China
The present study aimed to identify the composition of the aerial parts of Rubia cordifolia L. A chemical investigation on the EtOAc extracts from the aerial parts of Rubia cordifolia resulted in the isolation of four new anthraquinones, namely Cordifoquinone A–D (1–4), along with 16 known anthraquinones. Their structures were elucidated on the basis of NMR and HR-ESIMS data. All isolates were assessed for their inhibitory effects on NO production in LPS-stimulated RAW 264.7 macrophage cells. Compounds 1, 3 and 10 exhibited significant inhibitory activities with IC50 values of 14.05, 23.48 and 29.23 μmol·L−1, respectively. Their antibacterial activities of four bacteria, Escherichia coli (ATCC 25922), Staphylococcus aureus subsp. aureus (ATCC 29213), Salmonella enterica subsp. enterica (ATCC 14028) and Pseudomonas aeruginosa (ATCC 27853), were also evaluated. Our results indicated that the antibacterial activity of these compounds is inactive.
