Strain-specific identification of Beauveria bassiana isolated from a novel habitat, using rDNA-based sequence analogy

Abstract

Abstract Various branches of any living species are destined to specified identification. Henceforth, imposing of bioinformatics, including various supportive software along with wet lab experiments, has led to conclude genus and species of unidentified strains. Further, for the construction of phylogenetic trees, the principle of minimum evolution has been considered. Since few years, rapid sequencing of DNA has been adapted, and data on nucleotide sequences of genome in diverse organisms have accumulated at an accelerated pace. In the present study, molecular identification of the fungus, Beauveria bassiana, isolated from the soil of a tea cultivation area was endeavoured. Soil from Bogidhola tea garden of Golaghat District, Assam, India, has captured an attention as it holds some natural pesticidal properties. Since B. bassiana possesses substantial pesticidal property, an attempt was carried out to identify the presence of B. bassiana in that particular soil of tea garden, followed by its molecular identification. Sequencing of D2 subunit spanning 28S rDNA of isolated fungi had been completed for molecular identification imposing bioinformatic tools and among 15 isolates compared where a correction of 100% identification was observed with isolate of B. bassiana strain CCFC225554.

Keywords

• Bioinformatics
• BLAST
• DNA sequencing
• Entomopathogenic fungi
• Beauveria bassiana
• Phylogenetic tree