Serum-free cultured meat production by using Pichia pastoris-derived recombinant albumin
Ye Rim Kim,
Doyoung Hwang,
Si Won Jang,
Jae Ho Han,
Ga Rim Na,
Ji Hoon Park,
Gyeongho Tae,
Seung Moon Park,
Hyun Woo Choi
Affiliations
Ye Rim Kim
Department of Animal Science, Jeonbuk National University, Jeonju 54896, Republic of Korea
Doyoung Hwang
Department of Animal Science, Jeonbuk National University, Jeonju 54896, Republic of Korea; YEASTECHBIO Co., Ltd, Jeonju 54896, Republic of Korea
Si Won Jang
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea
Jae Ho Han
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea
Ga Rim Na
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea
Ji Hoon Park
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea
Gyeongho Tae
YEASTECHBIO Co., Ltd, Jeonju 54896, Republic of Korea
Seung Moon Park
YEASTECHBIO Co., Ltd, Jeonju 54896, Republic of Korea; Department of Bioenvironmental Chemistry, Jeonbuk National University, Jeonju 54896, Republic of Korea; Corresponding author
Hyun Woo Choi
Department of Animal Science, Jeonbuk National University, Jeonju 54896, Republic of Korea; Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea; Corresponding author
Summary: Cultured meat production involves the growth, differentiation, and food processing of muscle satellite cells. However, its heavy reliance on fetal bovine serum (FBS) significantly increases production costs, which has prompted interest in developing alternative serum substitutes. In this study, we aimed to enhance the economic viability and scalability of cultured meat production by cultivating and differentiating bovine muscle satellite cells (bMuSCs) in media supplemented with Pichia pastoris (P. pastoris)-derived bovine recombinant albumin (Br-A) or porcine recombinant albumin (Pr-A). As a result, P. pastoris-derived recombinant albumin (rAlbumin) effectively supported the proliferation of bMuSCs while maintaining their differentiation potential. In addition, the expression of the satellite cell marker Paired-box7 (Pax7) was upregulated. Moreover, P. pastoris-derived rAlbumin effectively induced myotube formation in the differentiation process. These results highlight the potential of P. pastoris-derived rAlbumin as a cost-effective and scalable alternative to serum components, providing a promising solution for serum-free media in the cultured meat industry.
