Plotter Cut Stencil Masks for the Deposition of Organic and Inorganic Materials and a New Rapid, Cost Effective Technique for Antimicrobial Evaluations
Andre Childs,
Jorge Pereira,
Charles M. Didier,
Aliyah Baksh,
Isaac Johnson,
Jorge Manrique Castro,
Edwin Davidson,
Swadeshmukul Santra,
Swaminathan Rajaraman
Affiliations
Andre Childs
Department of Material Science and Engineering, University of Central Florida, Orlando, FL 32816, USA
Jorge Pereira
Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA
Charles M. Didier
Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32827, USA
Aliyah Baksh
Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32827, USA
Isaac Johnson
Department of Mechanical and Aerospace Engineering, University of Central Florida, Orlando, FL 32816, USA
Jorge Manrique Castro
Department of Electrical and Computer Engineering, University of Central Florida, Orlando, FL 32816, USA
Edwin Davidson
Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA
Swadeshmukul Santra
Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA
Swaminathan Rajaraman
Department of Material Science and Engineering, University of Central Florida, Orlando, FL 32816, USA
Plotter cutters in stencil mask prototyping are underutilized but have several advantages over traditional MEMS techniques. In this paper we investigate the use of a conventional plotter cutter as a highly effective benchtop tool for the rapid prototyping of stencil masks in the sub-250 μm range and characterize patterned layers of organic/inorganic materials. Furthermore, we show a new diagnostic monitoring application for use in healthcare, and a potential replacement of the Standard Kirby-Bauer Diffusion Antibiotic Resistance tests was developed and tested on both Escherichia coli and Xanthomonas alfalfae as pathogens with Oxytetracycline, Streptomycin and Kanamycin. We show that the reduction in area required for the minimum inhibitory concentration tests; allow for three times the number of tests to be performed within the same nutrient agar Petri dish, demonstrated both theoretically and experimentally resulting in correlations of R ≈ 0.96 and 0.985, respectively for both pathogens.
