Nature Communications (Nov 2022)
Integration of single-cell transcriptomes and biological function reveals distinct behavioral patterns in bone marrow endothelium
- Young-Woong Kim,
- Greta Zara,
- HyunJun Kang,
- Sergio Branciamore,
- Denis O’Meally,
- Yuxin Feng,
- Chia-Yi Kuan,
- Yingjun Luo,
- Michael S. Nelson,
- Alex B. Brummer,
- Russell Rockne,
- Zhen Bouman Chen,
- Yi Zheng,
- Angelo A. Cardoso,
- Nadia Carlesso
Affiliations
- Young-Woong Kim
- Department of Stem Cell Biology and Regenerative Medicine, Gehr Family Center for Leukemia Research, Beckman Research Institute, City of Hope
- Greta Zara
- Department of Stem Cell Biology and Regenerative Medicine, Gehr Family Center for Leukemia Research, Beckman Research Institute, City of Hope
- HyunJun Kang
- Department of Stem Cell Biology and Regenerative Medicine, Gehr Family Center for Leukemia Research, Beckman Research Institute, City of Hope
- Sergio Branciamore
- Department of Computational and Quantitative Medicine, Division of Mathematical Oncology, Beckman Research Institute, City of Hope
- Denis O’Meally
- Center for Gene Therapy, Beckman Research Institute, City of Hope
- Yuxin Feng
- Division of Experimental Hematology and Cancer Biology, Cincinnati Children’s Hospital Medical Center
- Chia-Yi Kuan
- Department of Neuroscience, Center for Brain Immunology and Glia (BIG), University of Virginia School of Medicine
- Yingjun Luo
- Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope
- Michael S. Nelson
- Light Microscopy Core, Beckman Research Institute, City of Hope
- Alex B. Brummer
- Department of Computational and Quantitative Medicine, Division of Mathematical Oncology, Beckman Research Institute, City of Hope
- Russell Rockne
- Department of Computational and Quantitative Medicine, Division of Mathematical Oncology, Beckman Research Institute, City of Hope
- Zhen Bouman Chen
- Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope
- Yi Zheng
- Division of Experimental Hematology and Cancer Biology, Cincinnati Children’s Hospital Medical Center
- Angelo A. Cardoso
- Center for Gene Therapy, Beckman Research Institute, City of Hope
- Nadia Carlesso
- Department of Stem Cell Biology and Regenerative Medicine, Gehr Family Center for Leukemia Research, Beckman Research Institute, City of Hope
- DOI
- https://doi.org/10.1038/s41467-022-34425-z
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 18
Abstract
Here Kim et al. show that primary BMECs can be maintained ex vivo as distinct sinusoidal- and arterial-like populations and that the presence of macrophages is critical to preserve their native transcriptomic profiles and functional heterogeneity.
