Genetic identification for Staphylococcus aureus by using nuc gene

Abstract

To examine the recovered strains phenotypically by conventional methods and genotypically by using synthetic oligonucleotides of 21 and 24 bases in polymerase chain reaction to amplify a sequence of nuc gene , which encoded thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from isolated DNA, PCR products was detected by agarose gel electrophoresis. Our results showed that 100% of Staphylococcus aureus were positive to nuc gene .The PCR for amplification of nuc gene has potential for rapid diagnosis of S. aureus infections by direct testing of clinical specimens .

Keywords

• nuc gene