BOEC–Exo Addition Promotes In Vitro Maturation of Bovine Oocyte and Enhances the Developmental Competence of Early Embryos
Yiran Wei,
Muhammad Idrees,
Tabinda Sidrat,
Myeondon Joo,
Lianguang Xu,
Jonghyeok Ko,
Ilkeun Kong
Affiliations
Yiran Wei
Department of Animal Science, Division of Applied Life Science, Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju, Gyeongnam 52828, Korea
Muhammad Idrees
Department of Animal Science, Division of Applied Life Science, Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju, Gyeongnam 52828, Korea
Tabinda Sidrat
Department of Animal Science, Division of Applied Life Science, Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju, Gyeongnam 52828, Korea
Myeondon Joo
Department of Animal Science, Division of Applied Life Science, Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju, Gyeongnam 52828, Korea
Lianguang Xu
Department of Animal Science, Division of Applied Life Science, Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju, Gyeongnam 52828, Korea
Jonghyeok Ko
The King Kong Corp. Ltd., Gyeongsang National University, Jinju, Gyeongnam 52828, Korea
Ilkeun Kong
Department of Animal Science, Division of Applied Life Science, Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju, Gyeongnam 52828, Korea
Exosomes are nano-sized vesicles with abundant nucleic acids, proteins, lipids, and other regulatory molecules. The aim of this study was to examine the effect of BOEC–Exo on bovine in vitro oocyte maturation and in vitro embryo development. We found that a 3% Exo supplementation to IVM media significantly enhanced the oocyte maturation and reduced the accumulation of ROS in MII-stage bovine oocytes. Oocyte maturation related genes (GDF9 and CPEB1) also confirmed that 3% Exo treatment to oocytes significantly (p p p < 0.05) enhancement in the lipid metabolism. Finally, the oocytes matured in the presence of 3% Exo showed a significantly higher rate of embryo development and better implantation potential. Finally, we concluded that Exo positively influenced bovine oocyte in vitro maturation and improved the early embryo’s developmental competence.
