The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds

Abstract

Sugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than in untreated material, resulting in more than two times higher release of reducing sugars. In the investigated range of solids load, supplementation of fungal cellulases cocktail by beta-glucosidase increased production of reducing sugars from substrates, while 0.25 U/g was sufficient to achieve the highest effect under applied conditions.[ Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31002]

Keywords

• enzymatic hydrolysis
• beta-glucosidase
• cellulose
• hydrothermal pre-treatment
• sugar beet shreds