Knockout Serum Replacement as a Substitute for Exosome Free Serum

Abstract

Exosomes, extracellular vesicles secreted from cells, are carriers containing proteins, lipids, and nucleic acids. By adhering and releasing their contents to the recipient cells, exosomes play a major role in cellular communication. Based on a variety of their originated cells, cargos, and physiologic state of releasing cell, Exosomes have been attributed an extended range of roles such as signal transduction, reprogramming, epigenetic modification, and inflammation. Recently, it has been shown that exosomes are also involved in pathological mechanisms of diseases, such as neurodegenerative diseases, tumors, chronic inflammation, and cardiovascular diseases. Moreover, exosomes have been used as diagnostic markers for different diseases since their cargos and contents can reflect the physiological or pathological processes of the cells of origin. Because of having features of being carriers, they can be considered as a vehicle to drug delivery or gene therapy.

Keywords

• exosome
• fetal bovine serum
• knockout serum replacement
• purification