Unravelling the antioxidant behaviour of self-assembly β-Sheet in silk fibroin
Zhiyong Qian,
Chang Sun,
Qianqian Li,
Yafan Xie,
Lingpeng Zhan,
Xiangli Liu,
Guanbo Wang,
Yen Wei,
Juhui Qiu,
Qin Peng
Affiliations
Zhiyong Qian
Department of Anatomy the Basic Medicine College, Inner Mongolia Medical University, Hohhot, 010000, Inner Mongolia, China; Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China
Chang Sun
Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China
Qianqian Li
Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China
Yafan Xie
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, China
Lingpeng Zhan
Institute for Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China
Xiangli Liu
Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China
Guanbo Wang
Institute for Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China
Yen Wei
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China; School of Materials Science and Engineering, North Minzu University, Yinchuan, 750021, China; Corresponding author. The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 750021, China.
Juhui Qiu
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, China; Corresponding author. Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, China.
Qin Peng
Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China; Corresponding author.
Local oxidative stress in diseases or injury severely hinders cell homeostasis and organ regeneration. Antioxidant therapy is an effective strategy for oxidative stress treatment. Biomaterials with good biocompatibility and reactive oxygen species (ROS) scavenging ability are good choices for antioxidant therapeutics. However, there are few natural biomaterials that are identified with both biocompatibility and strong antioxidant activity. Here, we show, for the first time, that silk fibroin (SF) is a strong antioxidant, which can eliminate ROS in both cells and zebrafish. We further demonstrate that the β-sheet structures turn into a random coiled structure when SF is treated with hydrogen peroxide. The content of β-sheet structures can be increased by heating, thus enhancing the antioxidation properties of SF. Therefore, SF can serve as a good antioxidant biomaterial for therapeutics, and its β-sheet structure-based antioxidation mechanism provides a novel theoretical basis, which could be a new cue for more antioxidant biomaterial discovery and identification.
