Application of Single Extracellular Vesicle Analysis Techniques

Abstract

Junquan Zhu, Feifeng Wu,* Cuifang Li,* Jueyi Mao,* Yang Wang,* Xin Zhou,* Haotian Xie,* Chuan Wen Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chuan Wen, Department of Pediatrics, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha, Hunan, 410011, People’s Republic of China, Tel [phone], Email [email protected]: Extracellular vesicles (EVs) are lipid containers that are actively released by cells and contain complex molecular cargoes. These cargoes include abundant material such as genomes and proteins from cells of origin. They are involved in intercellular communication and various pathological processes, showing excellent potential for diagnosing and treating diseases. Given the significant heterogeneity of EVs in complex physiopathological processes, unveiling their composition is essential to understanding their function. Bulk detection methods have been previously used to analyze EVs, but they often mask their heterogeneity, leading to the loss of valuable information. To overcome this limitation, single extracellular vesicle (SEV) analysis techniques have been developed and advanced. These techniques allow for analyzing EVs’ physical information and biometric molecules at the SEV level. This paper reviews recent advances in SEV detection methods and summarizes some clinical applications for SEV detection strategies.Keywords: single extracellular vesicle analysis, heterogeneity, detection methods, disease diagnosis, new therapies

Keywords

• single extracellular vesicle analysis
• heterogeneity
• detection methods
• disease diagnosis
• new therapies