iScience (Mar 2020)
Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection
- Smruti Pushalkar,
- Bidisha Paul,
- Qianhao Li,
- Jian Yang,
- Rebeca Vasconcelos,
- Shreya Makwana,
- Juan Muñoz González,
- Shivm Shah,
- Chengzhi Xie,
- Malvin N. Janal,
- Erica Queiroz,
- Maria Bederoff,
- Joshua Leinwand,
- Julia Solarewicz,
- Fangxi Xu,
- Eman Aboseria,
- Yuqi Guo,
- Deanna Aguallo,
- Claudia Gomez,
- Angela Kamer,
- Donna Shelley,
- Yindalon Aphinyanaphongs,
- Cheryl Barber,
- Terry Gordon,
- Patricia Corby,
- Xin Li,
- Deepak Saxena
Affiliations
- Smruti Pushalkar
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Bidisha Paul
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Qianhao Li
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Jian Yang
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Rebeca Vasconcelos
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Shreya Makwana
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Juan Muñoz González
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Shivm Shah
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Chengzhi Xie
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Malvin N. Janal
- Department of Epidemiology and Health Promotion, New York University College of Dentistry, New York, NY 10010, USA
- Erica Queiroz
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Maria Bederoff
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Joshua Leinwand
- S. Arthur Localio Laboratory, Department of Surgery, New York University School of Medicine, New York, NY 10016, USA
- Julia Solarewicz
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Fangxi Xu
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Eman Aboseria
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Yuqi Guo
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Deanna Aguallo
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Claudia Gomez
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Angela Kamer
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Donna Shelley
- Department of Public Health Policy Analysis Management, New York University School of Global Public Health, New York, NY 10012, USA
- Yindalon Aphinyanaphongs
- Department of Population Health, New York University School of Medicine, New York, NY 10016, USA; Department of Medicine, New York University School of Medicine, New York, NY 10016, USA
- Cheryl Barber
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA
- Terry Gordon
- Department of Environmental Medicine, New York University School of Medicine, New York, NY 10016, USA
- Patricia Corby
- Department of Oral Medicine, University of Pennsylvania, School of Dental Medicine, Philadelphia, PA 19104, USA
- Xin Li
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA; Basic Science and Craniofacial Biology, Dental Center, 421 First Avenue, Room 901D, New York, NY, USA; Corresponding author
- Deepak Saxena
- Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, 345 East 24th Street, Room 921B, New York, NY 10010, USA; Corresponding author
- Journal volume & issue
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Vol. 23,
no. 3
Abstract
Summary: The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection. : In Vitro Toxicology; Microbiome; Oral Microbiology Subject Areas: In Vitro Toxicology, Microbiome, Oral Microbiology
